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  • 產(chǎn)品名稱：活體轉(zhuǎn)基因--鉗狀電極

  • 產(chǎn)品型號：BTX MODEL 524
  • 產(chǎn)品廠商：各類電極/緩沖液
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活體轉(zhuǎn)基因--鉗狀電極用于體內(nèi)藥/基因/蛋白質(zhì)傳遞及基因療法．活體轉(zhuǎn)基因--鉗狀電極Tweezertrodes電極是一種可復(fù)用的小鉗狀電極，主要用于體內(nèi)的藥或基因的傳遞。活體轉(zhuǎn)基因--鉗狀電極是一個(gè)標(biāo)準(zhǔn)的11.5厘米的小鉗子，其尖部為嵌入的不銹鋼圓盤狀電極，兩盤的間隙可調(diào)至2厘米，并有一個(gè)陽極指示器。
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活體轉(zhuǎn)基因--鉗狀電極用于體內(nèi)藥/基因/蛋白質(zhì)傳遞及基因療法電極．


活體轉(zhuǎn)基因--鉗狀電極Tweezertrodes是一種可復(fù)用的小鉗狀電極，主要用于體內(nèi)的藥或基因的傳遞。電極是一個(gè)標(biāo)準(zhǔn)的11.5厘米的小鉗子，其尖部為嵌入的不銹鋼圓盤狀電極，兩盤的間隙可調(diào)至2厘米，并有一個(gè)陽極指示器。


活體轉(zhuǎn)基因--鉗狀電極Tweezertrodes電極有兩種規(guī)格，45-0118為直徑：7mm的電極盤，45-0119為直徑：10mm的電極盤

當(dāng)把感興趣的分子注射到體內(nèi)后，用電極盤夾住相關(guān)組織，然后給出電脈沖，使孔道開始形成，將分子整合到直接與電極盤接觸的組織細(xì)胞內(nèi)。

Tweezertrodes are reusable, tweezer-style in vivo electrodes fordrug or gene delivery in animals. Tweezertrodes consist of astandard 11.5 cm tweezer that has been modified with stainlesssteel circular or disk electrodes at the tip. The gap between theelectrode disks may be adjusted from under 1 mm to over 2 cm.Tweezertrodes come in two models. Model 520 has an electrode diskdiameter of 7 mm and Model 522 has a diameter of 10 mm.Tweezertrodes may be cleaned with a mild detergent and sterilizedwith ethanol or ethylene oxide. These electrodes are connected to apulse generator with the Model 524 Connection Cable, and arecompatible with most BTX Electroporation System
Applications

Tweezertrodes may be used for many in vivo applications, includinggene and drug delivery. Following localized or systemic injectionof the molecule of interest, the Tweezertrode electrode disks areused to grasp the tissue of interest. An electroporation pulse isthen given, initiating pore formation and incorporation of themolecule into the cells of the tissue in direct contact with theelectrode disks. Suzuki et al. used Tweezertrodes to deliver aGreen Fluorescent Protein (GFP) gene into rat liver. The authorsdemonstrated successful gene transfer and expression in situ.1,2Saito and Nakatsuji used a similar electrode to introduce plasmidDNA during ex-utero and in-utero electroporation.7 Sato et al. usedthe Tweezertrode with the T820 to introduce EGFP expressing plasmidin mouse testis.8 Tweezertrodes complement BTX Genetrode Models508, 510, Caliper Electrode Models 384 and 384L, 2-Needle ArrayModels 530 and 532 as the newest addition to our in vivo electrodeline. The Tweezertrodes can be used to reproduce applicationsrequiring the caliper electrodes, including gene therapy,transdermal drug delivery and electroporation therapy.3,4,5,6

Technical Specifications


Standard Capabilities
Voltage Range: 0V - 200V (Do not use AC current)
Pulse Length Range: 1 μsec - 200 msec
Physical Characteristics
Model 520
Tweezertrode Length: 11.5 cm
Electrode Diameter: 7 mm
Electrode Material: Stainless steel
Model 522
Tweezertrode Length: 11.5 cm
Electrode Diameter: 10 mm
Electrode Material: Stainless steel
Generator Compatibility ECM T820, 630, 830, 2001

Ordering Information

System Model Available Configurations
520 Tweezertrode 520
Tweezer Electrode with stainless steel plated 7 mm diameterelectrode disk for in vivo applications

523 Tweezertrode 523
Tweezer Electrode with stainless steel plated 10mm diameterelectrode disk for in vivo applications

Accessory Model Description
524 Tweezertrode Connection Cable

References
Suzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivoElectroporation, FEBS Letters, 425: 436-440 (1998)
BTX Electroporation Protocol PR0363 (1998)
Zhang et al., In vivo transdermal delivery of large molecules bypressure-mediated electroincorporation and electroporation: a novelmethod for drug and gene delivery, Bioelectrochemistry andBioenergetics, 42: 283-292 (1997)
Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotidartery by electroporation, Third U.S. / Japan Symposium on DrugDelivery Systems (Abstr.), 38 (1995)
Dev, Killing cancer cells with a combination of pulsed electricfields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)
BTX Electroporation Therapy Protocols ECT001-ECT005 (1995)
Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)
Sato et al., Molecular Reproduction and Development, 61: 49-56(2002)
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